Part:BBa_K2347000

DHAP/G3P->Acrylic Acid

CEAS, which is N2- (2-carboxyethyl) arginine synthase, is an enzyme in Streptomyces clavuligerus, EC 2.5.1.66. CEAS is a TPP-related enzyme, can catalyze the condensation of D-G3P and L-Arg with the involvement of TPP and magnesium ions (thiamine pyrophosphate) to produce N2- (2-carboxyethyl) arginine, which will continue to participate in the biosynthesis of clavulanic acid as the first intermediate.

According to the earlier literature, CEAS (N2-(2-carboxyethyl) arginine synthase) was a synergistic effect of Ceas1 and Ceas, namely, N2- (2-carboxyethyl) arginine came from the condensation of G3P and L-Arg, which was catalyzed by Ceas1 and Ceas2. The process would be accompanied by the formation of acrylic acid[1]. But with the deepening of the study, Matthew E.C. Caines found that Ceas2 played the main role in the catalytic process. And they speculated the catalytic mechanism of Ceas2, as shown below [2].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 558
  Illegal AgeI site found at 211
  Illegal AgeI site found at 268
  Illegal AgeI site found at 477
  Illegal AgeI site found at 579
  Illegal AgeI site found at 805
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1304